发明名称 |
Plasmid, method for construction of the same, microorganisms carrying the plasmid and method for cultivation of the microorganism |
摘要 |
A novel plasmid pEAP2, which was constructed from Bacillus sp. 170 chromosomal DNA carrying the gene for extracellular production of penicillinase and a vector plasmid pMB9. A novel microorganism, Escherichia coli HB101(pEAP2) carrying the plasmid pEAP2 and being capable of extracellular production of penicillinase. The method for cultivation of the microorganism is characterized by culturing it in the medium containing NaCl (or KCl) for 16-48 hours. According to this invention, useful high-molecular substances can be produced in a high yield.
|
申请公布号 |
US4962055(A) |
申请公布日期 |
1990.10.09 |
申请号 |
US19870032032 |
申请日期 |
1987.03.30 |
申请人 |
RIKAGAKU KENKYUSHO |
发明人 |
HORIKOSHI, KOKI;KUDO, TOSHIAKI;KATO, CHIAKI |
分类号 |
C12N1/21;C12N1/38;C12N9/24;C12N9/86;C12N15/70 |
主分类号 |
C12N1/21 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|