| 摘要 |
A specific binding assay is described, having a matrix which provides for incorporation of a defined volume of liquid sample, two or more electrodes, a reversibly inactivated enzyme, a first binding partner specific for binding with the analyte in the sample, and a second binding partner which competes with the analyte for binding to the first binding partner or binds to the analyte, which is labeled with an agent capable of reversing the reversible inactivation. Upon hydration with a sample, the analyte and second binding partner compete for binding with the first binding partner. Labeled binding partner which does not bind to the immobilized binding partner is able to diffuse to the enzyme, where it reactivates the enzyme and thus produces an electrical signal. |
