ASSAY OF FREE AND COMPLEXED PROSTATE-SPECIFIC ANTIGEN (PSA)

摘要

According to the method of the invention, immunoassays are applied to measure free PSA as well as a proteinase inhibitor complex. Free PSA and PSA complex are according to the invention measured by a non-competitive immunoassay employing at least two different monoclonal antibodies. The invention is further characterized by that the PSA proteinase inhibitor complex of interest is formed either with alpha 1-antichymotrypsin, alpha 1-protease inhibitor (API) or alpha 2-macroglobulin. Moreover, the invention is characterized by that free PSA, the PSA-proteinase inhibitor complex and their ratio are applied in the diagnosis of patients with prostate cancer.