ALKALINE PROTEASE 3733, ITS PRODUCTION AND USE IN CLEANING CONTACT LENS

摘要

PCT/US 92/02597 31 The fermentation and isolation of a novel alkaline protease, protease 3733 is described. This enzyme has the properties of an elastase and a serine protease. This enzyme is produced by a newly discovered alkalophilic Bacillus sp. IAM 011105 bacterium. Protease 3733 is characterized by high activity in hydrolyzing denatured lysozyme and the use of this enzyme in cleaning contact lenses is described. The biochemical properties of the new enzyme are described and the activity of the enzyme on a variety of substrates under a variety of conditions is described and compared to other alkalineproteases.