DNA sequences coding for protein components of african horsesickness virus.

摘要

DNA sequences coding for protein components of African horsesickness virus are provided, as well as the use thereof in the expression of polypeptides. The DNA sequences and expressed polypeptides are useful as diagnostic tools and in the case of the expressed polypeptides, as vaccine components. The DNA sequences of the invention were deririved by synthesising cDNA by reverse transcribing AHSV RNA, producing a library of cDNA clones from said synthesised cDNA, and screening said library for clones coding for full length AHSV proteins. The screening procedure included the step of identifying clones containing DNA inserts having the 3'-sequence CATTCAC and/or the 5 min -sequence GTTAAAA.