LIGAND-STIMULATED GENE EXPRESSION

摘要

Disclosed is a method of producing a complementary DNA (cDNA) library enriched in ligand-inducible genes of a cell. The method includes activating a cellular ligand receptor for a predetermined period of time in the presence of labelled RNA precursors and a substance which enhances the level of RNA in said cell. The RNA precursors are incorporated into the RNA synthesized by said cell in response to receptor activation. The labelled RNA is separated from unlabelled RNA and used to prepare cDNA. The cDNA is cloned into host cells to provide a first cDNA library of cDNA-containing clones, which is then screen for clones containing ligand-induced genes. Clones containing ligand-inducible genes are selected to produce a second cDNA library enriched in ligand-inducible genes.