VERFAHREN ZUR ENZYMATISCHEN HYDROLYSE VON ALPHA-AMINOADIPINYL-MONOAMINOVERBINDUNGEN.

摘要

New gamma-glutamyl transpeptidase (GTP) has the following characteristics (a) mol. wt. 40,000-80,000; (b) isoelectric point at pH 4.4-5.9; (c) optimum pH 6.5-10, with Km = 9-36 micromolar at pH 8, for hydrolysis of l-gamma-glutamyl p-nitroanilide; (d) ability to hydrolyse alpha-aminoadipamic acid derivs. of formula (I), where R1 is the residue of an amino acid, dipeptide, cephem cpd. or cepham cpd. Use of GTP for hyrolysis of adipoyl or glutaryl monoamides is disclosed in EP-275901. GTP is produced by culturing a suitable Pseudomonas, Proteus, Arthrobactor or Bacillus strain, esp. B.subtilis IFO 3025, as described in EP275901. Hydrolysis of (I) is pref. effected at 28-35 deg.C and pH 6.6-8.0.