发明名称 |
DNA DETECTION METHOD |
摘要 |
A process for the detection of a nucleic acid sequence in a homogeneous assay format using an energy transfer system is disclosed. This process utilizesa 5' labeled primer containing a selfcomplementary sequence in an amplification process together with a subsequent detection step using a 3' labeled probe for the amplified region. The labels will be close together in space after hybridizing the probe dose to the short piece of double-stranded DNA resulting from backfolding of the selfcomplementary region of the primer which has been incorporated into the amplified product. The new primer for use in this process is also disclosed.
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申请公布号 |
CA2090904(A1) |
申请公布日期 |
1993.09.24 |
申请号 |
CA19932090904 |
申请日期 |
1993.03.03 |
申请人 |
F. HOFFMAN-LA ROCHE AG |
发明人 |
BANNWARTH, WILHELM;MUELLER, FRANCIS |
分类号 |
C07H21/00;C12N15/09;C12Q1/68;G01N33/50;(IPC1-7):C12Q1/68;C07H23/00;C07F9/11 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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