RECOMBINANT CELLS, DNA CONSTRUCTS, VECTORS AND METHODS FOR EXPRESSION PHYTATE DEGRADING ENZYMES IN DESIRED RATIOS

摘要

A recombinant combination strain which is capable of over-expressing at least two different genes under two separate promoters in filamentous fungi. The genes encode phytase and pH 2.5 acid phosphatase. GA- and GAPDH-promoters are preferably used. Mixtures containing desired ratios of the two enzymes are prepared by recombinant DNA techniques. The enzyme mixture shows a cooperative effect in the degradation of phytic acid and its salts. Preferred ratios of the two enzymes are about 3:1 to about 16:1. The DNA sequences were isolated from a wild-type (Aspergillus niger) ALKO243 (ATCC#38854).