METHOD, ARTICLE, AND APPARATUS FOR CRYOPRESERVATION OF BIOLOGICAL SAMPLES

摘要

Described herein is a method of storing a biological sample, such as a sperm sample, in an elongated container for cryopreservation. Multiple volumes of biologic al sample may be placed in each elongated container such that each volume of sample is separated from its nearest neighbor by a separation gas. The present invention further relates to an apparatus and method for loading biological samples, such as sperm samples, into an elongated container for cryopreservation of the sample material. The apparatus and method employs drawing the sample into the elongated container such that individual sample volumes are interspaced by gaps of separation gas. This alternating pattern may be creat ed by repeatedly contacting and removing the elongated container from the sample under suctio n pressure. The apparatus further incorporates the use of a computer to control the volu me and number of aliquots as desired. A container according to such methods is also provided.