发明名称 |
METHOD OF MEASUREMENT OF CELLULAR HEMOGLOBIN |
摘要 |
A method of measuring cellular hemoglobin of a blood sample includes mixing a blood sample with a permeation reagent, and incubating the sample mixture to permeate cellular membrane of red blood cells and to cause hemoglobin aggregation within the cells; adding a neutralization reagent to inhibit further reactions of the permeation reagent; performing a cell-by-cell measurement of side scatter signals of the red blood cells in the sample mixture on a flow cytometer; and obtaining cellular hemoglobin (Hgb<SUB>cell</SUB>) of each red blood cell using the obtained side scatter signals. The method further includes measuring cellular hemoglobin of reticulocytes (Hgb<SUB>retic</SUB>) by differentiating reticulocytes using a simultaneous fluorescence measurement. The method also includes measuring cellular percentage of a hemoglobin variant in mature red blood cells or reticulocytes by adding a fluorescent antibody in the neutralization reagent and detecting fluorescence signals of antibody bound hemoglobin variant. |
申请公布号 |
WO2008021653(A3) |
申请公布日期 |
2008.10.23 |
申请号 |
WO2007US73870 |
申请日期 |
2007.07.19 |
申请人 |
BECKMAN COULTER, INC. |
发明人 |
VAN AGTHOVEN, ANDREAS;MALERGUE, FABRICE |
分类号 |
G01N33/53 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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