| 摘要 |
The invention relates to a method and an assembly for generating optical split images. The invention permits the three-dimensional, layered optical scanning of spatially extended objects and is used in microscopy, but is not limited to this field. In said method, periodically distributed exposures are projected in one spatial direction into a plane and fluorescent and/or luminescent light that is reflected and/or scattered and/or emitted by the sample is reproduced on a local-resolution detector. According to the invention, a calibration step is first carried out, in which step the local phase and/or the local period of the distributed exposures are determined for each location on the detector. In the sample detection mode, two distributed exposures are projected into or onto the sample to calculate each split image and the resultant intensity distributions are reproduced on the detector. The method steps for projecting and detecting two exposure light distributions are repeated as often as desired, in particular for different focal positions of the sample and/or different exposure wavelengths and the intensity distributions are used to calculate at least one optical split image from the captured intensity distributions with the aid of the local phase and/or local period. |
