| 摘要 |
The present invention provides a DNA encoding a chimeric protein that comprises one transmembrane domain and one extracellular Fc-binding domain and provides transgenic myeloma cells and transgenic mice having the DNA integrated into their genome. The invention also provides methods of preparing transgenic myeloma cell and transgenic mice expressing the chimeric protein. The invention further provides methods of using the transgenic myeloma cells and transgenic mice said mice to facilitate isolation of monoclonal antibodies-producing B-cells. Specifically, antibody-producing cells are engineered to express a chimeric protein that comprises a transmembrane domain and an extracellular Protein G' domain, rendering the cells binding to antibodies released by themselves. Such character simplifies the subsequent selection of monoclonal antibody-producing cells.
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