METHOD FOR MODIFYING A PEPTIDE AND METHOD FOR IDENTIFYING A PEPTIDE

摘要

DISCLOSURE OF THE INVENTION The present invention is to provide a method for easily and specifically modifying specific amino acid residue(s) constituting a peptides and to provide a methodology of improving the accuracy of identification of the peptide using a new information of the peptide obtained from the number of modified amino acid residue by said specific modification method as mentioned. The method for modifying a peptide according to the present invention is characterized :
A method for modifying a peptide, wherein the peptide as supported in a substrate and an aqueous solution of perhalogenated carboxylic acid containing an alcohol is reacted to selectively esterify a glutamic acid residue of said peptide.
The method for identifying a peptide according to the present invention is characterized :
A method for identifying a peptide comprising the steps of:
reacting the peptide as supported in a substrate and an aqueous solution of perhalogenated carboxylic acid containing an alcohol to selectively esterify glutamic acid residue of said peptide;
immersing said substrate in a protease solution to obtain a peptide fragment originated from said peptide;
measuring a molecular weight of said peptide fragment; and
determining said peptide based on said molecular weight.