发明名称 |
ELECTROROTATION |
摘要 |
A method of analysis comprises forming complexes between micro-particles (12) such as plastics beads, a linking moiety (14) such as an antibody attached to the micro-particle, and a target (16) such as a micro-organism and observing the electro-rota-tion properties of the complex (10). Electro-rotation is produced by applying a rotating electric field in a plane transverse to the direction of observation. The differing electro-rotation properties of the micro-particles (12) coated with antibody (14) alone and the complexes (10) are visually observable and in suitable cases discrimination is possible between complexes containing live tar-get micro-organisms and those containing dead micro-organisms. The target (16) may be bound to a labelling moiety (14) such as a gold or magnetic particle to enhance change in electro-rotation properties produced on complex formation. A wide range of tar-get species are detectable, including nucleic acids and proteins.
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申请公布号 |
CA2129729(C) |
申请公布日期 |
2008.10.07 |
申请号 |
CA19932129729 |
申请日期 |
1993.02.05 |
申请人 |
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发明人 |
PARTON, ADRIAN;PETHIG, RONALD;BURT, JULIAN |
分类号 |
G01N33/543;C12Q1/68;C40B30/00;C40B70/00;G01N15/10;G01N27/00;G01N33/487;G01N33/53;G01N33/542;G01N33/569;G01N33/58 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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