发明名称 METHOD FOR ESTIMATING DNA CONCENTRATION AND METHOD FOR DETECTING DNA AMPLIFICATION
摘要 PROBLEM TO BE SOLVED: To provide a DNA amplification detecting method capable of immediately detecting whether amplification exists or not in a reaction tube after amplification reaction without requiring treatment of electrophoresis, etc. SOLUTION: The method for detecting DNA amplification comprises advancing DNA amplification reaction in the presence of microcapsules each including an aqueous dye solution specifically reacting with DNA in a paraffin wax having a melting point higher than a temperature of DNA amplification reaction, releasing the aqueous dye solution specifically reacting with DNA from microcapsules by heating the reaction solution after DNA amplification reaction and detecting existence or absence or the degree of existence of DNA amplification, based on color change of the reaction solution. COPYRIGHT: (C)2008,JPO&INPIT
申请公布号 JP2008194019(A) 申请公布日期 2008.08.28
申请号 JP20070042794 申请日期 2007.02.22
申请人 NATIONAL AGRICULTURE & FOOD RESEARCH ORGANIZATION;NIIGATA UNIV 发明人 OKUDA MITSURU;IWANAMI TORU;TANAKA MASATO
分类号 C12Q1/68;G01N33/50 主分类号 C12Q1/68
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