In-vitro test kit for animal experiment-free determination of sensitizing potential of substance, has coproculture of human keratinocyte and human monocyte

摘要

The in-vitro test kit has a coproculture of human keratinocyte and human monocytes. The monocyte is generated during the coproculture of cytokine interleukin-4 (IL-4), granulocyte monocyte colony stimulating factor (GM-CSF) and transforming growth factor (TGF)beta by dendritic cells. The coprocultured arborescent cells locker overlie on the adherent keratinocyte or float in the cell culture medium. An independent claim is also included for a method for manufacturing an in-vitro test kit for animal experiment-free determination of the sensitizing potential of a substance.