PROCESS AND REACTION REAGENT FOR OLIGONUCLEOTIDE SYNTHESIS AND PURIFICATION

摘要

PROBLEM TO BE SOLVED: To provide a method for removing amino protective groups from oligonucleotide.

SOLUTION: The method comprises mixing a polyamine, PEHA, PEG-NH2, a short PEG-NH2, a cycloalkylamine, a hydroxycycloalkylamine, a hydroxyamine, K2CO3/MeOH microwave, a thioalkylamine, a thiolated amine,β-amino-ethyl-sulfonic acid or sodium sulphate ofβ-amino-ethylsulfonic acid, with an oligonucleotide having amide protective groups.

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