Waschverfahren zur Entfernung unerwünschter Komponenten in zu analysierenden Proben

摘要

A method for removing an undesired component from a bound desired component in an analysis that includes the steps of: (a) providing a container having a desired component bound thereto and an undesired component; (b) dispensing a wash fluid in the container at a first level sufficient to contact at least a portion of the bound desired component; (c) oscillating the level of the wash fluid in the container; and (d) removing at least a portion of the wash fluid from the container. Another aspect of the invention provides a method for washing an analyte bound to the walls of a surface coated container of an analyzer that includes: (a) providing a surface coated container having an analyte bound thereto; (b) dispensing a wash fluid in the container at a first level sufficient to contact at least a portion of the analyte bound thereto; (c) oscillating the level of the wash fluid in the container; and (d) removing the wash fluid from the surface coated container. Also described is a method for removing an undesired component from a bound desired component in an analysis that includes the steps of: (a) providing a container having a desired bound component bound thereto and an undesired component; (b) dispensing a wash fluid in the container at a first level sufficient to wash at least a portion of the bound desired component; (c) removing the wash fluid from the container; (d) subsequently dispensing a wash fluid in the container at a subsequent level that is lower than the first level and is sufficient to wash at least a portion of the bound desired substrate; and (e) removing the wash fluid from the container. In a preferred embodiment, the analyte being measured is Troponin I.