发明名称 |
Screening using polarization anisotropy in FRET emissions |
摘要 |
Methods and apparatus are described for detecting specific binding between first and second chemical entities. The first chemical entity in association with a first fluorophore is immobilized. The second chemical entity is allowed to bind with the immobilized first chemical entity. The second chemical entity is or becomes coupled to a second fluorophore, which forms a FRET pair with the first fluorophore. The bound chemical entities are exposed to radiation at an excitation frequency for either the first or the second fluorophore, and polarization anisotropy of a FRET fluorescent signal from the bound chemical entities is measured to detect specific binding between the first and second chemical entities. Techniques are also disclosed for detecting whether a FRET interaction is occurring between a first chemical entity including a donor fluorophore and a second chemical entity including an acceptor fluorophore, using simultaneous anisotropy measurements at the wavelengths of the donor and acceptor fluorophores.
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申请公布号 |
US2008206888(A1) |
申请公布日期 |
2008.08.28 |
申请号 |
US20080069724 |
申请日期 |
2008.02.11 |
申请人 |
BLUESHIFT BIOTECHNOLOGIES, INC. |
发明人 |
MILLER STEVEN C.;COMITA PAUL B.;SHUMATE CHRISTOPHER B.;CROMWELL EVAN F. |
分类号 |
G01N33/566;G01N21/76 |
主分类号 |
G01N33/566 |
代理机构 |
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代理人 |
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地址 |
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