发明名称 |
VERFAHREN ZUR PROTEINISOLIERUNG UNTERANOXISCHEN BEDINGUNGEN |
摘要 |
The present invention relates to a method for the isolation of proteins that comprise disulfide-bonds in their native conformation. Essentially, a method of the present makes the use of reducing agents such as &bgr;-mercaptoethanol or dithiothreitol in protein isolation methods obsolete. A method of the present invention is particularly suitable for the isolation of precursor proteins such as proinsulin from recombinant cells. |
申请公布号 |
AT397619(T) |
申请公布日期 |
2008.06.15 |
申请号 |
AT20040706187T |
申请日期 |
2004.01.29 |
申请人 |
N.V. ORGANON |
发明人 |
VAN DER MEIJDEN, PETER;VAN DEDEM, GIJSBERT;EPPINK, MICHEL;WASSENAAR, ROELAND |
分类号 |
C07K1/113;C07K1/36;C07K14/62 |
主分类号 |
C07K1/113 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|