发明名称 |
Methods and compositions for targeted cleavage and recombination |
摘要 |
<p>Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.</p> |
申请公布号 |
AU2004263865(B2) |
申请公布日期 |
2007.05.17 |
申请号 |
AU20040263865 |
申请日期 |
2004.08.06 |
申请人 |
SANGAMO BIOSCIENCES, INC. |
发明人 |
JEFFREY C. MILLER;FYODOR URNOV;MICHAEL C. HOLMES;CARL O. PABO |
分类号 |
C12P19/34;C07K14/47;C12N;C12N9/22;C12N15/10 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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