Influenza virus sub-unit vaccine

摘要

1,140,316. Influenza vaccine. AUSTRALIAN NATIONAL UNIVERSITY. 22 Feb., 1967 [24 Feb., 1966; 7 April, 1966], No. 8458/67. Heading A5B. Influenza vaccine is prepared by treating an aqueous suspension of influenza virus particles with an aqueous solution of a soluble salt of desoxycholic acid, whereby the particles are split into sub-units, and then reducing the concentration of the said salt in the vaccine to a non-toxic level. The preferred salt is sodium desoxycholate which is added to the suspension to give a concentration of 0.5 to 2.0% wt./vol., the concentration subsequently being reduced to below 0.01%, e.g. by dialysis. The treatment may be performed at 20‹-37‹C, at pH 7.5-8.2, and takes from 1 to 60 minutes. Virus strains used are swine (SW) strain 15 of shope, BEL strain, Asian AS(A2/AA/23/57), and two Australian strains, A2/SA/64 and B/Vic/65; they are grown up in embryonate egg culture prior to treatment.