发明名称 |
Methods for detecting nucleic acid variations |
摘要 |
The invention provides methods and diagnostic test kits for detecting target nucleic acid sequence variations in a sample. In particular, a plurality of oligonucleotide probe sets is provided. Each set has a target specific portion and a barcode. The target specific portions of the probes are suitable for ligation together when hybridized adjacent to one another on a corresponding target polynucleotide. The ligated product contains a barcode that binds to a probe attached to a substrate and hence facilitate the capture of the ligated product on the solid support. Detection is carried out with a gold nanoparticle-attached barcode that hybridizes with the barcode on the ligated product.
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申请公布号 |
US2007111204(A1) |
申请公布日期 |
2007.05.17 |
申请号 |
US20040562415 |
申请日期 |
2004.06.25 |
申请人 |
DELGROSSO KATHLEEN;FORTINA PAOLO;GRAVES DAVID;SURREY SAUL |
发明人 |
DELGROSSO KATHLEEN;FORTINA PAOLO;GRAVES DAVID;SURREY SAUL |
分类号 |
C12Q1/68;C07H21/04;C12P19/34;C12Q |
主分类号 |
C12Q1/68 |
代理机构 |
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地址 |
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