METHOD FOR MEASURING CONCENTRATION OF NUCLEIC ACID AND ITS COMPONENTS, CONCENTRATION-MEASURING DEVICE AND SYNTHETIC DEVICE HAVING THE SAME

摘要

PROBLEM TO BE SOLVED: To directly calculate the concentration of a deoxynucleotide or the like in a measuring object solution from an ultraviolet absorption spectrum of the measuring object solution.

SOLUTION: The concentration of each of the deoxynucleotide in the measuring object solution can directly be calculated from the ultraviolet absorption spectrum of the measuring object solution with respect to each deoxynucleotide aqueous solution by subjecting the relation between the concentration of the deoxynucleotide and the ultraviolet absorbance at 220-300 nm by 1 nm unit of the sampling width to PLS regression analysis. The method can be applied even to an oligomer, a single stranded DNA and a double stranded DNA, and can calculate each concentration thereof.

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