Encapsulation of substances in liposome comprises applying two different monolayers on porous carrier membrane; applying different fluid volumes on monolayers; bringing compressible membrane and lacing the dismantled area to liposome

摘要

Encapsulation of substances in liposome (1) (which is bilayer (11) made from monomolecular layers (3,4)) comprises applying the two different monolayers on the surface of a porous carrier membrane (2); applying different fluid volumes on the outer side of the monolayers; bringing a compressible membrane (6) (arranged with e.g. pores) in contact with e.g. the bilayer; distorting the bilayer; and lacing the dismantled area (12) to the liposome. Encapsulation of substances (preferably active substance) in liposome (1) (which is bilayer (11) made from monomolecular layers (3,4)) comprises applying the two different monolayers on the surface of a porous carrier membrane (2) (where both monolayers, based on physical interactions between the substances of them, form the bilayer with freely adjustable composition on the internal and external side); applying different fluid volumes (5, 7, 9, 10) (preferably in thin film form) on the respective outer side of the monolayers surrounding the exterior of the prepared liposome or storing within the prepared liposome; bringing a compressible membrane (6) (arranged with pores (48) and channels filled with buffer medium adjacent on the inside of the liposome where the fluid volume comes in contact) in contact with the bilayer arrangement, fluid volumes and porous carrier membrane; distorting the bilayer (in the pore of the porous membrane by an over pressure (8) in the buffer medium) so that the bilayer is pressed into the pores and contacts with the fluid volume stored within the liposome at least partially tearing itself and dismantling by the layer; and lacing the dismantled area (12) of the layer (due to minimization of the boundary surface energy) to a liposome and simultaneously wrapping the fluid volume containing the substance to be stored in the liposome by the porous carrier membrane. Independent claims are also included for: (1) a discontinuously working device, for encapsulation or substances in the liposome, preferably for carrying out the above method, comprising a planar carrier stretched on the carrier membrane and at least one roller successive to the different membranes and fluid volumes, where on one roller the carrier membrane is brought into contact with other membrane by relative motion; and (2) a continuously working device, for encapsulating substances in liposomes and preferably for carrying out the above method, comprising a cylindrical carrier stretched on the porous carrier membrane and an arrangement of different membranes and fluid volumes rolling on each other (by rotatory relative motions) on the shifting rollers and transferred on the porous carrier membrane and also bringing in contact with it.