| 摘要 |
The invention relates to methods of making anti-ligand libraries, which anti-ligands have a cavity binding site. The method comprises providing a plurality of anti-ligands having a cavity for binding a ligand, said plurality of anti-ligands being derived from a first anti-ligand having a cavity for binding a first ligand and differing from the first anti-ligand in that one or more of the amino acid residues which make up the ligand binding surface of the cavity are varied whereby the plurality of anti-ligands have a binding affinity for a first ligand which is different from that of the first anti-ligand, and wherein the one or more amino acid residues which is varied, is varied by replacement with one amino acid having similar but different physicochemical properties to the one or more amino acid residues, and wherein the one or more amino acids being varied can be varied to become a different amino acid in each anti-ligand molecule being varied. Preferably the anti-ligands are antibodies or fragments thereof which are capable of forming a cavity which can bind a ligand.
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