| 摘要 |
A novel gene defining a novel enzyme UDP-galactose: beta-D-galactose-R 4-alpha-D-galactosyltransferase, termed alpha4Gal-T1, with unique enzymatic properties is disclosed. The invention provides isolated DNA molecules and DNA constructs encoding alpha4Gal-T1 and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting alpha4Gal-T1 activity, as well as cloning and expression vectors including such DNA, host cells comprising DNA encoding alpha4Gal-T1, and recombinant methods for providing alpha4Gal-T1. The enzyme alpha4Gal-T1 and alpha4Gal-active derivatives thereof are disclosed. Further, the invention discloses methods of obtaining alpha1, 4galactosyl glycosylated glycosphingolipids by use of an enzymatically active alpha4Gal-T1 protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active alpha4Gal-T1 protein as an expression system for recombinant production of such glycosphingolipids. Also a method for the identification of DNA sequence variations in the alpha4Gal-T1-coding exon by PCR, and detecting the presence of DNA sequence variation, are disclosed.
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