发明名称 |
TRANSFORMED FILAMENTOUS FUNGI, METHOD FOR OBTAINING THEREOF, AND METHODS OF EXPRESSING AND OBTAINING PROTEINS WHEN USING THEM |
摘要 |
The invention provides a method for the expression of exogenous DNA libraries in filamentous fungi. The fungi are capable of processing intron-containing eukaryotic genes, and also can carry out post-translational processing steps such as glycosylation and protein folding. The invention provides for the use of fungi with altered morphology, which permits high-throughput screening and directed molecular evolution of expressed proteins. The same transformed fungi may be used (as shown in figure 1) to produce larger quantities of protein for isolation, characterization, and application testing, and may be suitable for commercial production of the protein as well. |
申请公布号 |
EA006873(B1) |
申请公布日期 |
2006.04.28 |
申请号 |
EA20020000035 |
申请日期 |
2001.04.13 |
申请人 |
EMALFARB MARK, AARON |
发明人 |
PUNT PETER, JAN;VAN ZEIJL CORNELIA;VAN DEN HONDEL CORNELIUS |
分类号 |
C12N15/09;C12Q1/68;C07H21/02;C12N1/00;C12N1/14;C12N15/00;C12N15/10;C12P21/02;C12Q1/04;C12R1/645 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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