| 摘要 |
Methods of electrochemically monitoring enzyme-substrate reactions are described. The substrate is tagged with a redox-active group. Modification of the substrate by an enzyme can then be monitored electrochemically by means of the redox-active group. Such methods can be used in particular to monitor non-redox enzyme reactions, such as kinase, phosphatase, or protease reactions, although redox enzyme reactions can also be monitored by such methods. Screening assays to identify modulators of enzyme activity or new enzyme substrates are also described. |
