摘要 |
Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit "single-tube" processing. |
申请人 |
BRANDEIS UNIVERSITY;WANGH, LAWRENCE, J.;RICE, JOHN;SANCHEZ, AQUILES, J.;PIERCE, KENNETH;SALK, JESSE;REIS, ARTHUR |
发明人 |
WANGH, LAWRENCE, J.;RICE, JOHN;SANCHEZ, AQUILES, J.;PIERCE, KENNETH;SALK, JESSE;REIS, ARTHUR |