DETECTION METHOD FOR TARGET PROTEIN USING APTAMER AND THE DETECTION KIT

摘要

A method for detecting a target protein using a DNA aptamer is provided to be more convenient and economical than a conventional method using an antibody and be used for detecting the target protein with high sensitivity through amplification of the fluorescence intensity by repetitive action of RNase H. A method for detecting a target protein comprises the steps of: (a) binding a DNA aptamer coupled to the target protein to a D-GNA(guard-DNA) complementary to the aptamer to form a double-stranded DNA; (b) treating a single stranded RNA, which is complementary to the double-stranded DNA and the G-DNA and is coupled to a fluorophore and a quencher, with a sample including the target protein; (c) treating RNase H; and (d) measuring the intensity of the fluorescence in the sample. A kit for detecting a target protein comprises: a double stranded DNA consisting of a DNA aptamer coupled to the target protein and a G-DNA; a single stranded RNA coupled to a fluorophore and a quencher; and RNase H. Further, the DNA aptamer is 5'Ex_aptamer.