发明名称 |
Process for Purification of Human Growth Hormone |
摘要 |
Adding enough organic solvent to hydrophobic interactive chromatography elution buffer eliminates the need to also add detergent to the buffer when separating poly-peptides. For example, adding about 50% acetonitrile to detergent-free elution buffer enaibls one to separate full-length human growth hormone from its various truncated forms, to obtain hGH with a purity of >99.5%. This technique is useful to purify polypeptide where detergent to contamination in the resulting polypeptide is undesirable.
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申请公布号 |
US2008167452(A1) |
申请公布日期 |
2008.07.10 |
申请号 |
US20070568235 |
申请日期 |
2007.10.29 |
申请人 |
MAITI DIPANWITA;MISHRA SHRIKANT;RAO LAXMI SRINIVAS;NIPHADKAR MILIND PRABHAKAR;BORBHUIYA AHMED MONSUR;RAO MADHAVA YADA |
发明人 |
MAITI DIPANWITA;MISHRA SHRIKANT;RAO LAXMI SRINIVAS;NIPHADKAR MILIND PRABHAKAR;BORBHUIYA AHMED MONSUR;RAO MADHAVA YADA |
分类号 |
C07K16/00 |
主分类号 |
C07K16/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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