MICROPATTERNING OF BIOMOLECULES

摘要

The present invention concerns methods and compositions for detecting, monitoring, or measuring various intracellular processes and molecular interactions. In one embodiment, the present invention provides a method for assessing a molecular interaction comprising: (a) providing a cell comprising a labeled target molecule and a membrane moiety having an extracellular domain; (b) providing a patterned surface comprising a pattern of interaction areas, the interaction areas comprising immobilized ligands specific to the extracellular domain of the membrane bound moiety; (c) contacting the cell with the patterned surface; and (d) detecting a signal from the labeled target molecule, wherein a greater signal at the interaction areas of the patterned surface as compared to areas between the interaction areas indicates a molecular interaction between the labeled target molecule and the membrane bound moiety.