| 摘要 |
The invention provides a method of isolating nucleic acid from biological material, the method comprising the steps: a) providing biological material suspended in a solution, b) adjusting the pH of the suspension from a) to within the range about pH 1 to about pH 6, c) contacting nucleic acid in the suspension from b) with an affinity matrix capable of binding the nucleic acid in the pH range about pH 1 to about pH 6, in order to bind the nucleic acid to the affinity matrix; and d) eluting the nucleic acid from the affinity matrix using a buffer with a pH of more than 8, wherein the method does not require a centrifugation or filtration step prior to step c). |
