METHODS FOR PRODUCING AN ADENOVIRUS TYPE 5 GENE TRANSFER VECTOR

摘要

pZerotgCMV, an Ad-5-based expression vector in current use for gene transfer stimulates lipogenic enzymes within HepG2 cells (human hepatic carcinoma cells) and primary rat hepatocytes in vitro. Evidence indicates increased lipid accumulation in infected cells compared to uninfected cells. Therefore, inactivation of the E4 ORF1 gene or E4 gene cluster whether by replacement, removal, mutation, or use of antisense RNA encoded by the Ad-5 genome may prevent activation of lipogenic genes and subsequent lipid accumulation. Removal of just the E4 gene from pZerotgCMV may prevent both replication and stimulation of lipogenic enzymes.