| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a simple assay for detecting cAMP (cyclic adenosine monophosphate formed upon modulation of adenylyl cyclase activity), and an in vitro method for indirectly detecting receptor activity and for screening of ligands. <P>SOLUTION: The in vitro method for detecting cAMP or cGMP comprises: (a) contacting a mixture with a complex of a tracer and a dequencher, wherein the tracer is a fluorophore covalently linked to cAMP quencher; and (b) measuring the change in fluorescence. Furthermore, the method is used for determining the cAMP or cGMP concentration in the mixture, for determining the activity of a receptor wherein the signal transduction of the receptor comprises cAMP or cGMP, and for screening of a ligand for such a receptor. <P>COPYRIGHT: (C)2008,JPO&INPIT</p> |
