METHOD FOR DETECTING EXTENDED DNA STRAND AMPLIFIED BY PCR AND METHOD FOR DETERMINING TARGET SEQUENCE

摘要

PROBLEM TO BE SOLVED: To provide a new method for detecting a PCR amplification product enabling separate detection of a plurality of amplification products in the same reaction liquid in high quantitative accuracy. SOLUTION: The target sequence is used as a template, and a primer and a labeled probe are annealed with the template. A DNA strand complementary to the target sequence is extended in 5'→3' direction from the primer annealed to the target sequence by the polymerase activity of the DNA polymerase, and the labeled probe annealed to the target sequence is decomposed by the 5'→3' exonuclease activity of the DNA polymerase to isolate a fragment bound with the labeled substance. The extension reaction (and the accompanying decomposition reaction) is repeated to amplify the extended DNA strand complementary to the labeled sequence. The reaction liquid is subjected to mass analysis to detect the presence and the quantity of the isolated fragments bound with the labeled substance and detect the presence or amplified quantity of the extended DNA strand complementary to the labeled sequence. COPYRIGHT: (C)2008,JPO&INPIT