发明名称 |
Methods for protein labeling based on acyl carrier protein |
摘要 |
Disclosed is a method for labeling acyl carrier protein (ACP) fusion proteins with a wide variety of different labels. The method relies on the transfer of a label from a coenzyme A type substrate to an ACP fusion protein using a holo-acyl carrier protein synthase (ACPS) or a homologue thereof. The method allows detecting and manipulating the fusion protein by attaching molecules to the fusion proteins that introduce a new physical or chemical property to the fusion protein. Examples of such labels are, among others, spectroscopic probes or reporter molecules, affinity tags, molecules generating reactive radicals, cross-linkers, ligands mediating protein-protein interactions or molecules suitable for the immobilization of the fusion protein.
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申请公布号 |
NZ543653(A) |
申请公布日期 |
2008.10.31 |
申请号 |
NZ20040543653 |
申请日期 |
2004.05.19 |
申请人 |
EPFL - ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE |
发明人 |
JOHNSSON, KAI;GEORGE, NATHALIE |
分类号 |
C07K14/245;G01N33/535;G01N33/58;(IPC1-7):G01N33/532;G01N33/533;G01N33/534;C07K19/00;C07K14/705 |
主分类号 |
C07K14/245 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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