发明名称 |
THE PURO-DHFR QUADRIFUNCTIONAL MARKER AND ITS USE IN PROTEIN PRODUCTION |
摘要 |
This invention relates to industrial production of proteins. More specifically, the invention relates to the res-DHFR surrogate marker, which corresponds to a fusion between DHFR and a protein conferring resistance to a toxic compound or conferring a metabolic advantage. The invention further relates to the use of res-DHFR for screening cells for high expression of a protein of interest. The invention is illustrated by the Puro-DHFR surrogate marker, which corresponds to a fusion between the puromycin N-acetyltransferase and dihydrofolate reductase (DHFR). |
申请公布号 |
WO2008148881(A1) |
申请公布日期 |
2008.12.11 |
申请号 |
WO2008EP57109 |
申请日期 |
2008.06.06 |
申请人 |
LABORATOIRES SERONO SA;KOBR, MICHEL;DUPRAZ, PHILIPPE |
发明人 |
KOBR, MICHEL;DUPRAZ, PHILIPPE |
分类号 |
C07K19/00;C12N5/10;C12N15/65;G01N33/50 |
主分类号 |
C07K19/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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