发明名称 |
ENZYME ACTIVITY ASSAY USING ROLLING CIRCLE AMPLIFICATION |
摘要 |
<p>The present invention relates to an enzyme activity assay using rolling circle amplification for verifying that a sample contains the enzyme activity in question. Thus, the present invention pertains to a method for determining the presence or absence of one or more enzyme activities involved in circularising a non-circular oligonucleotide probe in a biological sample. Furthermore, the present invention concerns liquid compositions comprising one or more oligonucleotide probes. Within the scope of the present invention is also a composition comprising a liquid composition and a tissue sample, and solid support of one or more oligonucleotides of the present invention. Disclosed is also a microfluidic device with one or more compartments for performing rolling circle amplification events, and a method for correlating one or more rolling circle amplification events. Methods for testing the efficacy of a drug, for diagnosing or prognosing a disease, for treating a disease, or for treating prophylactically a disease is furthermore disclosed.</p> |
申请公布号 |
WO2008148392(A1) |
申请公布日期 |
2008.12.11 |
申请号 |
WO2008DK50132 |
申请日期 |
2008.06.03 |
申请人 |
IN SITU RCP A/S;LOHMANN, JAKOB SCHWALBE;STOUGAARD, MAGNUS;KOCH, JOERN ERLAND |
发明人 |
LOHMANN, JAKOB SCHWALBE;STOUGAARD, MAGNUS;KOCH, JOERN ERLAND |
分类号 |
C12Q1/68;C12Q1/25 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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