TREATMENT OF PAIN BY SINGLE CHAIN, POLYPEPTIDE FUSION PROTEIN

摘要

Use of a therapeutic molecule for the manufacture of a medicament for the treatment of a particular type of pain, wherein the therapeutic molecule comprises a non-cytotoxic protein conjugate that binds to a nociceptive sensory afferent cell, and comprises: (i) a Targeting Moiety (TM), wherein said TM is an agonist of a receptor present on said nociceptive sensory afferent cell, and wherein said receptor undergoes endocytosis to be incorporated into an endosome within the nociceptive sensory afferent cell; (ii) a non-cytotoxic protease or a fragment thereof, wherein the protease or protease fragment is capable of cleaving a protein of the exocytic fusion apparatus of said nociceptive sensory afferent cell; and (iii) a Translocation Domain, wherein the Translocation Domain translocates the protease or protease fragment from within the endosome, across the endosomal membrane, and into the cytosol of the nociceptive sensory afferent cell.