发明名称 |
EXPRESSION OF SOLUBLE ANTIBODY FRAGMENT BY TRUNCATION OF CH1 DOMAIN |
摘要 |
Improved expression of active antibody fragments (Fabs) is achieved by truncating a heavy chain constant region. Truncation of the CHI domain of a Fab fragment can increase yield of soluble active antibody fragment in Pseudomonas fluorescens. Another embodiment of the invention includes secretion of the light chain and a fragment of the heavy chain with various C-termini (e.g., VH- CHI truncated to different lengths). The truncated CHI region can be used as a scaffold to create other Fabs. Also included is truncation of the kappa light chain and/or lambda light chain domains of a Fab fragment. The invention also includes expression of Fab fragments fused to other peptides or molecules (e.g., toxins, proteins, peptides, enzymes, etc.). |
申请公布号 |
WO2008151319(A2) |
申请公布日期 |
2008.12.11 |
申请号 |
WO2008US66187 |
申请日期 |
2008.06.06 |
申请人 |
DOW GLOBAL TECHNOLOGIES INC.;MITCHELL, JON, C.;RETALLACK, DIANE |
发明人 |
MITCHELL, JON, C.;RETALLACK, DIANE |
分类号 |
C07K16/00 |
主分类号 |
C07K16/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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