发明名称 |
SUPPRESSION OF RESISTANCE IN INSECTS TO BACILLUS THURINGIENSIS CRY TOXINS, USING TOXINS THAT DO NOT REQUIRE THE CADHERIN RECEPTOR |
摘要 |
The invention relates to a method for obtaining DNA constructions coding for Cry toxins with 3 domains (also known as Cry toxins, Bt toxins or d-endotoxins), which lack helix a-1. Said DNA constructions were modified in order to encode proteins that can kill insects that are resistant to the corresponding non-modified Cry toxins. The invention relates to the DNA constructions of the genes of modified 3-domain Cry toxins and the modified 3-domain Cry proteins, together with methods, molecular vectors and host cells containing said constructions, as well as recombinant methods for producing the modified 3-domain Cry toxins. In addition, the invention relates to formulations containing the modified 3-domain Cry toxins. Resistance of insects to non-modified Cry toxins is due to a reduced binding of the toxin to the receptors in the insect's intestine. More specifically, the invention relates to the expression of the modified 3-domain Cry toxins, the methods for expressing said modified 3-domain Cry toxins, transformed microorganisms and transgenic plants with constructions that express the modified 3-domain Cry toxins and methods for suppressing resistance of pests to non-modified Cry toxins. |
申请公布号 |
WO2008150150(A2) |
申请公布日期 |
2008.12.11 |
申请号 |
WO2007MX00068 |
申请日期 |
2007.06.08 |
申请人 |
UNIVERSIDAD NACIONAL AUTONOMA DE MEXICO;ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA;PARDO-LOPEZ, LILIANA;TABASHNIK, ELLIOT BRUCE;SOBERON-CHAVEZ, MARIO;BRAVO-DE LA PARRA, MARIA ALEJANDRA |
发明人 |
PARDO-LOPEZ, LILIANA;TABASHNIK, ELLIOT BRUCE;SOBERON-CHAVEZ, MARIO;BRAVO-DE LA PARRA, MARIA ALEJANDRA |
分类号 |
A01H5/00 |
主分类号 |
A01H5/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|