Assays using enzymatic degradation of RNA/DNA heteroduplexes are provided for the detection of target nucleic acid molecules. Such enzymatic degradation may be obtained by enzymes including RNaseH. Exemplary methods include Probe Trapping (PT), Hybrid Energy Transfer (HET), and Fluorescent Probe Degradation (FPD). The assays make use of an RNA or chimeric RNA/DNA probe which recognizes a target DNA sequence and forms an RNA/DNA heteroduplex that is the substrate for the enzyme RNaseH which degrades the RNA portion of the probe. Degraded probe fragments diffuse away from the DNA target leading to a detectable signal and allowing the DNA target to hybridize to another probe. Probe degradation is cycled over time. The assays disclosed herein are suitable for use in detecting DNA sequences and could be used in a medical diagnostic.
申请公布号
WO2007103558(A3)
申请公布日期
2008.12.11
申请号
WO2007US06041
申请日期
2007.03.09
申请人
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;REICH, NORBERT, O.;BRAUN, GARY;ESTABROOK, R., AUGUST
发明人
REICH, NORBERT, O.;BRAUN, GARY;ESTABROOK, R., AUGUST