发明名称 |
METHOD FOR SEPARATION AND IDENTIFICATION OF BIOMOLECULES USING UNCONVENTIONAL GEL ELECTROPHORESIS AND DETECTION OF SINGLE NANOPARTICLE PROBES |
摘要 |
A method for identifying and separating of target biomolecules is disclosed. The method involves contacting a sample with a nanoparticle probe that is capable of specifically binding a target biomolecule. The complex formed by the nanoparticle target biomolecule is separated by electrophoresis using a non- conventional gel composed of a synthetically cross-linked polymer and a gel stabilizer. The nanoparticle probe:target biomolecule complex is detected, thereby identifying the target biomolecule. In certain examples, the nanoparticle probe emits a signal to identify its location. Particular examples of nanoparticle probes are semiconductor nanocrystals, such as quantum dots, which fluoresce at a particular wavelength. Also disclosed is a method of substantially sealing and/or increasing the transparency of a membrane. Such a method includes applying a film to a membrane. Sealing the membrane allows the detection of single nanoparticles.
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申请公布号 |
WO2008051985(A3) |
申请公布日期 |
2008.12.11 |
申请号 |
WO2007US82255 |
申请日期 |
2007.10.23 |
申请人 |
OREGON HEALTH & SCIENCE UNIVERSITY;VU, TANIA, Q.;LIU, HONG, YAN |
发明人 |
VU, TANIA, Q.;LIU, HONG, YAN |
分类号 |
G01N33/543;B01D57/02;G01N33/561 |
主分类号 |
G01N33/543 |
代理机构 |
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地址 |
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