发明名称 |
A CYANINE-BASED PROBE/TAG-PEPTIDE PAIR FOR FLUORESCENCE PROTEIN IMAGING AND FLUORESCENCE PROTEIN IMAGING METHODS |
摘要 |
<p>A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCI3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.</p> |
申请公布号 |
WO2008127768(B1) |
申请公布日期 |
2008.12.11 |
申请号 |
WO2008US53600 |
申请日期 |
2008.02.11 |
申请人 |
BATTELLE MEMORIAL INSTITUTE;MAYER-CUMBLIDGE, ULJANA, M.;CAO, HAISHI |
发明人 |
MAYER-CUMBLIDGE, ULJANA, M.;CAO, HAISHI |
分类号 |
C09B23/06;C07K1/13;C09B23/08 |
主分类号 |
C09B23/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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