发明名称 |
Direct screening method |
摘要 |
The invention concerns a method for screening a repertoire of polypeptides to identify one or more members thereof which interact with one or more target molecules, comprising: a) immobilising the target molecule(s) on a support; b) arranging a plurality of nucleic acid molecules encoding the repertoire of polypeptides in an array; c) juxtaposing the target molecule(s) and the arrayed nucleic acid molecules; d) expressing the arrayed nucleic acid molecules to produce the polypeptides such that said polypeptides come into contact with the target molecules(s) on the support and a subset of the polypeptides interacts with the target molecules; and e) detecting the interaction of the polypeptides with the target molecules on the support. The invention also provides a high density antibody array consisting of thousands of different polypeptide features, spatially arranged on a solid support for screening against different target ligands
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申请公布号 |
EP2000804(A2) |
申请公布日期 |
2008.12.10 |
申请号 |
EP20080000334 |
申请日期 |
2000.12.04 |
申请人 |
DOMANTIS LIMITED |
发明人 |
HOLT, LUCY JESSICA;DE WILDT, RUDOLF MARIA THEODORA;TOMLINSON, IAN |
分类号 |
G01N31/22;G01N33/68;C12M1/34;C12N15/09;C12Q1/02;C12Q1/68;C40B30/04;G01N33/53;G01N33/566;G01N37/00 |
主分类号 |
G01N31/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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