| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for measuring an enzyme activity, capable of measuring using simpler operation, as compared with conventional methods, and further is capable of measuring the enzyme activity of the enzymes inside living tissues and living cells. SOLUTION: This method for measuring the enzyme activity for measuring the amount of a substrate metabolite formed by substrate metabolism with the enzyme, by detecting an irradiated wave formed by the multiphoton excitation process of the substrate or substrate metabolite is provided. Also the device for measuring the enzyme activity is provided, at least by equipping a laser light source 11 of a near-infrared femtosecond laser light inducing the multiphoton excitation process of the substrate or substrate metabolite; an irradiated wave-detecting part 31 for detecting the irradiated wave formed by the multiphoton excitation process of the substrate or substrate metabolite and an optical passage for introducing the near infrared femtosecond laser light to an enzyme-presenting site; and also introducing the irradiated waves to the irradiated wave-detecting part. COPYRIGHT: (C)2009,JPO&INPIT |
