REGULATION OF ApoB BY Hspl 10 PROTEINS AND RELATED COMPOSITIONS AND METHODS

摘要

Hspl 10 proteins are shown herein to stabilize nascent ApoB protein. Methods of down- regulating expression of ApoB, and thus down-regulating serum cholesterol levels, are therefore, provided, including, without limitation, inhibition of Hspl 10 expression by any method, for example and without limitation, by RNA interference (RNAi). It also has been found that ApoB gene-containing cells are useful in identifying compounds and genes that affect the expression of longer forms of ApoB. Lastly, a firefly luciferase-derived polypeptide is shown herein to bind the peptide binding site of Hspl 10, thus serving as a powerful analytical reagent for identifying compounds, such as small molecule compounds, that would interfere with Hspl 10-moderated stabilization of ApoB.